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    // var data = {"id":1,"parentId":0,"order":1,"children":null,"lang":"ZH","tags":null,"category":null,"formatContent":"\n<p>erghwg grewgrewgerwgerwg<\/p>\n","createDate":"2025-01-22T10:29:17.799+08:00","updateDate":"2025-01-22T10:41:47.887+08:00","templateName":"DEFAULT_ARTICLE","status":"PUBLISHED","likes":0,"visits":0,"commentNum":0,"summary":"erghwg grewgrewgerwgerwgerghwg grewgrewgerwgerwg","title":"test","viewName":"202502217816","path":"html\/news","picPath":null,"picThumbPath":"","pdfPath":null,"toc":"","categoryId":1,"tocJSON":"[]","commentTemplateName":"DEFAULT_COMMENT","openComment":true,"articleListSize":null,"isDesc":null,"linkPath":"\/news\/202502217816.html","top":false,"cssClass":null,"isDivision":false,"tagIds":null,"tocList":null,"isPublisher":false,"parentCategories":null,"forwardContentVO":null,"nextcontentVO":null,"css":"","js":"","originalContent":"erghwg grewgrewgerwgerwg","oldCategory":null,"commentPath":null,"articleInComponentOrder":0,"bilibili":"","youtube":"","video":"","categoryPath":"html\/news","categoryViewName":"index","isArticleDocLink":false,"users":null}
      // MARKDOWNB ROWSER
    // var data = { "parseType":"MARKDOWN","id": 9397, "parentId": 0, "order": 3, "children": null, "lang": "ZH", "tags": [{ "id": 36, "parentId": 0, "order": 0, "createDate": "2023-10-25T23:53:20.000+08:00", "updateDate": "2023-10-25T23:53:20.000+08:00", "lang": "ZH", "langSource": null, "name": "\u8F6C\u5F55\u7EC4", "description": null, "slugName": "\u8F6C\u5F55\u7EC4", "enName": "202392520195", "key": null, "url": null }], "category": null, "formatContent": "\n<p>\u53C2\u8003<br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01c_RNAseq_count_distribution.html\">Gene-level differential expression analysis<\/a><br\/>+ <a href=\"https:\/\/uclouvain-cbio.github.io\/WSBIM2122\/sec-rnaseq.html\">Differential expression analysis<\/a><br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html\">https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html<\/a><br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html\">https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html<\/a><\/p>\n<p><a href=\"https:\/\/github.com\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"https:\/\/img.shields.io\/badge\/github-bioproj%2Fbioinfo-brightgreen?logo=github\"><\/a> <a href=\"https:\/\/colab.research.google.com\/github\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"\/badage\/colab-badge.svg\"><\/a><\/p>\n<p>## Count matrix<br\/>\u5F53\u6211\u4EEC\u9700\u8981\u5F00\u59CB\u8FDB\u884CRNA-seq\u6570\u636E\u5206\u6790\u4E4B\u524D\uFF0C\u6211\u4EEC\u4F1A\u5F97\u5230\u4E00\u4E2A\u57FA\u56E0\u6C34\u54C1\u7684\u8868\u8FBE\u77E9\u9635\uFF0C\u5176\u4E2D\u884C\u662F\u57FA\u56E0\uFF0C\u5217\u662F\u6837\u672C\uFF0C\u77E9\u9635\u4E2D\u7684\u6BCF\u4E2A\u503C\u90FD\u662FCounts(\u6B63\u6574\u6570)\u3002<\/p>\n<p><div class=\"pic-warp\"><div class=\"data-original row wp-90\"><img class=\"lazy\" data-original=\"http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png\" alt=\"\u751F\u4FE1\u5C0F\u6728\u5C4B\" \/><\/div><\/div><\/p>\n<p>\u50CFt-test\u8FD9\u7C7B\u578B\u7684\u68C0\u9A8C\u4E0D\u662F\u4E3Acounts\u8BBE\u8BA1\u7684\uFF0C\u5E76\u4E14\u6211\u4EEC\u5728\u5B9E\u9A8C\u8BBE\u8BA1\u65F6\u4E00\u7EC4\u7684\u91CD\u590D\u901A\u5E38\u4E0D\u662F\u5F88\u5927\uFF0C\u6211\u4EEC\u4E0D\u80FD\u76F8\u4FE1\u4E2D\u5FC3\u6781\u9650\u5B9A\u7406(Central Limit Theorem)\u53BB\u4F7F\u7528t-test\uFF0C\u56E0\u6B64\u6211\u4EEC\u9700\u8981\u5BF9counts\u8FDB\u884C\u5EFA\u6A21\u3002<\/p>\n<p>\u4E3A\u4E86\u65B9\u4FBF\u540E\u7EED\u8BA8\u8BBA\uFF0C\u6211\u5C06\u5148\u4ECB\u7ECD\u4E00\u4E9B\u7B26\u53F7<br\/>+ <span class=\"bioinfo-katex\">X_{ij}<\/span>\u8868\u793A<strong>gene i<\/strong> (i=1,...,m)\u5728<strong>sample j<\/strong> (j=1,...,n)\u4E2D\u7684Count\uFF0C\u8FD9\u4E2ACount\u6765\u6E90\u4E8Ereads\u6BD4\u5BF9\u5230reference genome \u5BF9\u5E94\u57FA\u56E0\u533A\u57DF\u7684reads\u6570\u91CF\uFF0C\u8FD9\u91CC\u6211\u4EEC\u4E0D\u8BA8\u8BBA\u53EF\u53D8\u526A\u5207\uFF0C\u6211\u4EEC\u8BA4\u4E3A\u57FA\u56E0\u65F6\u4E00\u4E2A\u5355\u5143\u3002\u5F53\u7136\uFF0C\u5728reads mapping\u7684\u65F6\u5019\u6211\u4EEC\u53EF\u80FD\u8003\u8651\u5230\uFF0C\u662F\u5426\u65F6\u552F\u4E00\u7684mapping\uFF0C\u8FD9\u4E9B\u5728\u8FD9\u91CC\u90FD\u5FFD\u7565\u3002<\/p>\n<p>\u5F53\u6211\u4EEC\u8003\u8651count\u7684\u5206\u5E03\u65F6\uFF0C\u6211\u4EEC\u662F\u8BF4\u540C\u4E00\u7EC4\u5904\u7406\u6761\u4EF6\u4E0B\u7684gene\u90FD\u6709\u4E00\u4E2A\u5BF9\u5E94\u7684\u5206\u5E03\uFF0C\u56E0\u4E3A\u6BCF\u4E00\u4E2A\u57FA\u56E0\u90FD\u5F88\u72EC\u7279(\u6709\u81EA\u5DF1\u7684\u957F\u5EA6\u3001\u8868\u8FBE\u91CF)\uFF0C\u5F53\u6211\u4EEC\u8FDB\u884C\u5DEE\u5F02\u57FA\u56E0\u8868\u8FBE\u5206\u6790\u65F6\u6211\u4EEC\u662F\u5BF9\u6BCF\u4E00\u4E2A\u57FA\u56E0\u8FDB\u884C\u5047\u8BBE\u68C0\u9A8C\uFF0C\u5982\u679C\u5DEE\u5F02\u8868\u8FBE\u786E\u5B9E\u5B58\u5728\uFF0C\u90A3\u4E48\u4E24\u4E2A\u5904\u7406\u6761\u4EF6\u4E0B\u7684\u5206\u5E03\u5C06\u662F\u4E0D\u540C\u7684\u3002<\/p>\n<p>\u6211\u4EEC\u53EF\u4EE5\u7528\u7B26\u53F7<span class=\"katex\">X_{i1},...,X_{ij}\\sim Count Distribution<\/span>\u8868\u793A\u4E00\u4E2A\u6761\u4EF6\u4E0B\u7684\u4E00\u4E2A\u57FA\u56E0\u662F\u72EC\u7ACB\u540C\u5206\u5E03(Independent and identically distributed,iid)<\/p>\n<p>\u5BF9\u4E8E\u8FD9\u4E2ACount Distribution\u6211\u4EEC\u53EF\u4EE5\u8003\u8651poisson<\/p>\n", "createDate": "2025-01-19T01:32:51.000+08:00", "updateDate": "2025-01-19T11:39:56.000+08:00", "templateName": "DEFAULT_COURSE_TEMPLATE", "status": "PUBLISHED", "likes": 0, "visits": 3, "commentNum": 0, "summary": "\u53C2\u8003+ Gene-level differential expression analysis+ Differential expression analysis+ https:\/\/hbctraini....", "title": "test", "viewName": "202501950638", "path": "html\/articleList", "picPath": "http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png", "picThumbPath": "", "pdfPath": null, "toc": "", "categoryId": 94, "tocJSON": "[]", "commentTemplateName": "DEFAULT_COMMENT", "openComment": true, "articleListSize": null, "isDesc": null, "linkPath": "\/articleList\/202501950638.html", "top": false, "cssClass": null, "isDivision": false, "tagIds": [36], "tocList": null, "isPublisher": false, "parentCategories": null, "forwardContentVO": null, "nextcontentVO": null, "css": "", "js": "", "originalContent": "  \n\n    \u53C2\u8003\n    + [Gene-level differential expression analysis](https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01c_RNAseq_count_distribution.html)\n    + [Differential expression analysis](https:\/\/uclouvain-cbio.github.io\/WSBIM2122\/sec-rnaseq.html)\n    + <https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html>\n    + <https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html>\n    \n    \n    <a href=\"https:\/\/github.com\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"https:\/\/img.shields.io\/badge\/github-bioproj%2Fbioinfo-brightgreen?logo=github\"><\/a> <a href=\"https:\/\/colab.research.google.com\/github\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"\/badage\/colab-badge.svg\"><\/a>  \n    \n    ## Count matrix\n    \u5F53\u6211\u4EEC\u9700\u8981\u5F00\u59CB\u8FDB\u884CRNA-seq\u6570\u636E\u5206\u6790\u4E4B\u524D\uFF0C\u6211\u4EEC\u4F1A\u5F97\u5230\u4E00\u4E2A\u57FA\u56E0\u6C34\u54C1\u7684\u8868\u8FBE\u77E9\u9635\uFF0C\u5176\u4E2D\u884C\u662F\u57FA\u56E0\uFF0C\u5217\u662F\u6837\u672C\uFF0C\u77E9\u9635\u4E2D\u7684\u6BCF\u4E2A\u503C\u90FD\u662FCounts(\u6B63\u6574\u6570)\u3002\n    \n    ![html=(class=wp-90)\u751F\u4FE1\u5C0F\u6728\u5C4B](http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png)\n    \n    \u50CFt-test\u8FD9\u7C7B\u578B\u7684\u68C0\u9A8C\u4E0D\u662F\u4E3Acounts\u8BBE\u8BA1\u7684\uFF0C\u5E76\u4E14\u6211\u4EEC\u5728\u5B9E\u9A8C\u8BBE\u8BA1\u65F6\u4E00\u7EC4\u7684\u91CD\u590D\u901A\u5E38\u4E0D\u662F\u5F88\u5927\uFF0C\u6211\u4EEC\u4E0D\u80FD\u76F8\u4FE1\u4E2D\u5FC3\u6781\u9650\u5B9A\u7406(Central Limit Theorem)\u53BB\u4F7F\u7528t-test\uFF0C\u56E0\u6B64\u6211\u4EEC\u9700\u8981\u5BF9counts\u8FDB\u884C\u5EFA\u6A21\u3002\n    \n    \u4E3A\u4E86\u65B9\u4FBF\u540E\u7EED\u8BA8\u8BBA\uFF0C\u6211\u5C06\u5148\u4ECB\u7ECD\u4E00\u4E9B\u7B26\u53F7\n    + $X_{ij}$\u8868\u793A**gene i** (i=1,...,m)\u5728**sample j** (j=1,...,n)\u4E2D\u7684Count\uFF0C\u8FD9\u4E2ACount\u6765\u6E90\u4E8Ereads\u6BD4\u5BF9\u5230reference genome \u5BF9\u5E94\u57FA\u56E0\u533A\u57DF\u7684reads\u6570\u91CF\uFF0C\u8FD9\u91CC\u6211\u4EEC\u4E0D\u8BA8\u8BBA\u53EF\u53D8\u526A\u5207\uFF0C\u6211\u4EEC\u8BA4\u4E3A\u57FA\u56E0\u65F6\u4E00\u4E2A\u5355\u5143\u3002\u5F53\u7136\uFF0C\u5728reads mapping\u7684\u65F6\u5019\u6211\u4EEC\u53EF\u80FD\u8003\u8651\u5230\uFF0C\u662F\u5426\u65F6\u552F\u4E00\u7684mapping\uFF0C\u8FD9\u4E9B\u5728\u8FD9\u91CC\u90FD\u5FFD\u7565\u3002\n    \n    \n    \u5F53\u6211\u4EEC\u8003\u8651count\u7684\u5206\u5E03\u65F6\uFF0C\u6211\u4EEC\u662F\u8BF4\u540C\u4E00\u7EC4\u5904\u7406\u6761\u4EF6\u4E0B\u7684gene\u90FD\u6709\u4E00\u4E2A\u5BF9\u5E94\u7684\u5206\u5E03\uFF0C\u56E0\u4E3A\u6BCF\u4E00\u4E2A\u57FA\u56E0\u90FD\u5F88\u72EC\u7279(\u6709\u81EA\u5DF1\u7684\u957F\u5EA6\u3001\u8868\u8FBE\u91CF)\uFF0C\u5F53\u6211\u4EEC\u8FDB\u884C\u5DEE\u5F02\u57FA\u56E0\u8868\u8FBE\u5206\u6790\u65F6\u6211\u4EEC\u662F\u5BF9\u6BCF\u4E00\u4E2A\u57FA\u56E0\u8FDB\u884C\u5047\u8BBE\u68C0\u9A8C\uFF0C\u5982\u679C\u5DEE\u5F02\u8868\u8FBE\u786E\u5B9E\u5B58\u5728\uFF0C\u90A3\u4E48\u4E24\u4E2A\u5904\u7406\u6761\u4EF6\u4E0B\u7684\u5206\u5E03\u5C06\u662F\u4E0D\u540C\u7684\u3002\n    \n    \n    \u6211\u4EEC\u53EF\u4EE5\u7528\u7B26\u53F7$X_{i1},...,X_{ij}\\sim Count Distribution$\u8868\u793A\u4E00\u4E2A\u6761\u4EF6\u4E0B\u7684\u4E00\u4E2A\u57FA\u56E0\u662F\u72EC\u7ACB\u540C\u5206\u5E03(Independent and identically distributed,iid)\n    \n    \n    \u5BF9\u4E8E\u8FD9\u4E2ACount Distribution\u6211\u4EEC\u53EF\u4EE5\u8003\u8651poisson \n  ", "oldCategory": null, "commentPath": null, "articleInComponentOrder": 0, "bilibili": "", "youtube": "", "video": "", "categoryPath": "html\/articleList", "categoryViewName": "202501655577", "isArticleDocLink": false, "users": null }
    // var type="article"
    // var data = {"id":9397,"parentId":0,"order":32,"children":null,"lang":"ZH","tags":null,"category":null,"formatContent":null,"createDate":"2024-04-05T23:19:45.000+08:00","updateDate":"2025-01-22T22:53:17.000+08:00","templateName":"DEFAULT_ARTICLE","status":"MODIFY","likes":0,"visits":47,"commentNum":0,"summary":"\u53C2\u8003+ Gene-level differential expression analysis+ Differential expression analysis+ https:\/\/hbctraini....","title":"test","viewName":"20243544737","path":"html\/articleList","picPath":"http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png","picThumbPath":"","pdfPath":null,"toc":"","categoryId":94,"tocJSON":"[]","commentTemplateName":"DEFAULT_COMMENT","openComment":true,"articleListSize":null,"isDesc":null,"linkPath":"\/articleList\/20243544737.html","top":false,"cssClass":null,"isDivision":false,"tagIds":null,"tocList":null,"isPublisher":false,"parentCategories":null,"forwardContentVO":null,"nextcontentVO":null,"css":"","js":"","originalContent":null,"oldCategory":null,"commentPath":null,"articleInComponentOrder":0,"bilibili":"","youtube":"","video":"","categoryPath":"html\/articleList","categoryViewName":"202011184323","isArticleDocLink":null,"users":null}
    var data = { "name":"哲学","parseType":"MARKDOWN","id": 2462, "parentId": 0, "order": 3, "children": null, "lang": "ZH", "tags": [{ "id": 36, "parentId": 0, "order": 0, "createDate": "2023-10-25T23:53:20.000+08:00", "updateDate": "2023-10-25T23:53:20.000+08:00", "lang": "ZH", "langSource": null, "name": "\u8F6C\u5F55\u7EC4", "description": null, "slugName": "\u8F6C\u5F55\u7EC4", "enName": "202392520195", "key": null, "url": null }], "category": null, "formatContent": "\n<p>\u53C2\u8003<br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01c_RNAseq_count_distribution.html\">Gene-level differential expression analysis<\/a><br\/>+ <a href=\"https:\/\/uclouvain-cbio.github.io\/WSBIM2122\/sec-rnaseq.html\">Differential expression analysis<\/a><br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html\">https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html<\/a><br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html\">https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html<\/a><\/p>\n<p><a href=\"https:\/\/github.com\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"https:\/\/img.shields.io\/badge\/github-bioproj%2Fbioinfo-brightgreen?logo=github\"><\/a> <a href=\"https:\/\/colab.research.google.com\/github\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"\/badage\/colab-badge.svg\"><\/a><\/p>\n<p>## Count matrix<br\/>\u5F53\u6211\u4EEC\u9700\u8981\u5F00\u59CB\u8FDB\u884CRNA-seq\u6570\u636E\u5206\u6790\u4E4B\u524D\uFF0C\u6211\u4EEC\u4F1A\u5F97\u5230\u4E00\u4E2A\u57FA\u56E0\u6C34\u54C1\u7684\u8868\u8FBE\u77E9\u9635\uFF0C\u5176\u4E2D\u884C\u662F\u57FA\u56E0\uFF0C\u5217\u662F\u6837\u672C\uFF0C\u77E9\u9635\u4E2D\u7684\u6BCF\u4E2A\u503C\u90FD\u662FCounts(\u6B63\u6574\u6570)\u3002<\/p>\n<p><div class=\"pic-warp\"><div class=\"data-original row wp-90\"><img class=\"lazy\" data-original=\"http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png\" alt=\"\u751F\u4FE1\u5C0F\u6728\u5C4B\" \/><\/div><\/div><\/p>\n<p>\u50CFt-test\u8FD9\u7C7B\u578B\u7684\u68C0\u9A8C\u4E0D\u662F\u4E3Acounts\u8BBE\u8BA1\u7684\uFF0C\u5E76\u4E14\u6211\u4EEC\u5728\u5B9E\u9A8C\u8BBE\u8BA1\u65F6\u4E00\u7EC4\u7684\u91CD\u590D\u901A\u5E38\u4E0D\u662F\u5F88\u5927\uFF0C\u6211\u4EEC\u4E0D\u80FD\u76F8\u4FE1\u4E2D\u5FC3\u6781\u9650\u5B9A\u7406(Central Limit Theorem)\u53BB\u4F7F\u7528t-test\uFF0C\u56E0\u6B64\u6211\u4EEC\u9700\u8981\u5BF9counts\u8FDB\u884C\u5EFA\u6A21\u3002<\/p>\n<p>\u4E3A\u4E86\u65B9\u4FBF\u540E\u7EED\u8BA8\u8BBA\uFF0C\u6211\u5C06\u5148\u4ECB\u7ECD\u4E00\u4E9B\u7B26\u53F7<br\/>+ <span class=\"bioinfo-katex\">X_{ij}<\/span>\u8868\u793A<strong>gene i<\/strong> (i=1,...,m)\u5728<strong>sample j<\/strong> (j=1,...,n)\u4E2D\u7684Count\uFF0C\u8FD9\u4E2ACount\u6765\u6E90\u4E8Ereads\u6BD4\u5BF9\u5230reference genome \u5BF9\u5E94\u57FA\u56E0\u533A\u57DF\u7684reads\u6570\u91CF\uFF0C\u8FD9\u91CC\u6211\u4EEC\u4E0D\u8BA8\u8BBA\u53EF\u53D8\u526A\u5207\uFF0C\u6211\u4EEC\u8BA4\u4E3A\u57FA\u56E0\u65F6\u4E00\u4E2A\u5355\u5143\u3002\u5F53\u7136\uFF0C\u5728reads mapping\u7684\u65F6\u5019\u6211\u4EEC\u53EF\u80FD\u8003\u8651\u5230\uFF0C\u662F\u5426\u65F6\u552F\u4E00\u7684mapping\uFF0C\u8FD9\u4E9B\u5728\u8FD9\u91CC\u90FD\u5FFD\u7565\u3002<\/p>\n<p>\u5F53\u6211\u4EEC\u8003\u8651count\u7684\u5206\u5E03\u65F6\uFF0C\u6211\u4EEC\u662F\u8BF4\u540C\u4E00\u7EC4\u5904\u7406\u6761\u4EF6\u4E0B\u7684gene\u90FD\u6709\u4E00\u4E2A\u5BF9\u5E94\u7684\u5206\u5E03\uFF0C\u56E0\u4E3A\u6BCF\u4E00\u4E2A\u57FA\u56E0\u90FD\u5F88\u72EC\u7279(\u6709\u81EA\u5DF1\u7684\u957F\u5EA6\u3001\u8868\u8FBE\u91CF)\uFF0C\u5F53\u6211\u4EEC\u8FDB\u884C\u5DEE\u5F02\u57FA\u56E0\u8868\u8FBE\u5206\u6790\u65F6\u6211\u4EEC\u662F\u5BF9\u6BCF\u4E00\u4E2A\u57FA\u56E0\u8FDB\u884C\u5047\u8BBE\u68C0\u9A8C\uFF0C\u5982\u679C\u5DEE\u5F02\u8868\u8FBE\u786E\u5B9E\u5B58\u5728\uFF0C\u90A3\u4E48\u4E24\u4E2A\u5904\u7406\u6761\u4EF6\u4E0B\u7684\u5206\u5E03\u5C06\u662F\u4E0D\u540C\u7684\u3002<\/p>\n<p>\u6211\u4EEC\u53EF\u4EE5\u7528\u7B26\u53F7<span class=\"katex\">X_{i1},...,X_{ij}\\sim Count Distribution<\/span>\u8868\u793A\u4E00\u4E2A\u6761\u4EF6\u4E0B\u7684\u4E00\u4E2A\u57FA\u56E0\u662F\u72EC\u7ACB\u540C\u5206\u5E03(Independent and identically distributed,iid)<\/p>\n<p>\u5BF9\u4E8E\u8FD9\u4E2ACount Distribution\u6211\u4EEC\u53EF\u4EE5\u8003\u8651poisson<\/p>\n", "createDate": "2025-01-19T01:32:51.000+08:00", "updateDate": "2025-01-19T11:39:56.000+08:00", "templateName": "DEFAULT_COURSE_TEMPLATE", "status": "PUBLISHED", "likes": 0, "visits": 3, "commentNum": 0, "summary": "\u53C2\u8003+ Gene-level differential expression analysis+ Differential expression analysis+ https:\/\/hbctraini....", "title": "test", "viewName": "202501950638", "path": "html\/articleList", "picPath": "http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png", "picThumbPath": "", "pdfPath": null, "toc": "", "categoryId": 94, "tocJSON": "[]", "commentTemplateName": "DEFAULT_COMMENT", "openComment": true, "articleListSize": null, "isDesc": null, "linkPath": "\/articleList\/202501950638.html", "top": false, "cssClass": null, "isDivision": false, "tagIds": [36], "tocList": null, "isPublisher": false, "parentCategories": null, "forwardContentVO": null, "nextcontentVO": null, "css": "", "js": "", "originalContent": "  \n\n    \u53C2\u8003\n    + [Gene-level differential expression analysis](https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01c_RNAseq_count_distribution.html)\n    + [Differential expression analysis](https:\/\/uclouvain-cbio.github.io\/WSBIM2122\/sec-rnaseq.html)\n    + <https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html>\n    + <https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html>\n    \n    \n    <a href=\"https:\/\/github.com\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"https:\/\/img.shields.io\/badge\/github-bioproj%2Fbioinfo-brightgreen?logo=github\"><\/a> <a href=\"https:\/\/colab.research.google.com\/github\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"\/badage\/colab-badge.svg\"><\/a>  \n    \n    ## Count matrix\n    \u5F53\u6211\u4EEC\u9700\u8981\u5F00\u59CB\u8FDB\u884CRNA-seq\u6570\u636E\u5206\u6790\u4E4B\u524D\uFF0C\u6211\u4EEC\u4F1A\u5F97\u5230\u4E00\u4E2A\u57FA\u56E0\u6C34\u54C1\u7684\u8868\u8FBE\u77E9\u9635\uFF0C\u5176\u4E2D\u884C\u662F\u57FA\u56E0\uFF0C\u5217\u662F\u6837\u672C\uFF0C\u77E9\u9635\u4E2D\u7684\u6BCF\u4E2A\u503C\u90FD\u662FCounts(\u6B63\u6574\u6570)\u3002\n    \n    ![html=(class=wp-90)\u751F\u4FE1\u5C0F\u6728\u5C4B](http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png)\n    \n    \u50CFt-test\u8FD9\u7C7B\u578B\u7684\u68C0\u9A8C\u4E0D\u662F\u4E3Acounts\u8BBE\u8BA1\u7684\uFF0C\u5E76\u4E14\u6211\u4EEC\u5728\u5B9E\u9A8C\u8BBE\u8BA1\u65F6\u4E00\u7EC4\u7684\u91CD\u590D\u901A\u5E38\u4E0D\u662F\u5F88\u5927\uFF0C\u6211\u4EEC\u4E0D\u80FD\u76F8\u4FE1\u4E2D\u5FC3\u6781\u9650\u5B9A\u7406(Central Limit Theorem)\u53BB\u4F7F\u7528t-test\uFF0C\u56E0\u6B64\u6211\u4EEC\u9700\u8981\u5BF9counts\u8FDB\u884C\u5EFA\u6A21\u3002\n    \n    \u4E3A\u4E86\u65B9\u4FBF\u540E\u7EED\u8BA8\u8BBA\uFF0C\u6211\u5C06\u5148\u4ECB\u7ECD\u4E00\u4E9B\u7B26\u53F7\n    + $X_{ij}$\u8868\u793A**gene i** (i=1,...,m)\u5728**sample j** (j=1,...,n)\u4E2D\u7684Count\uFF0C\u8FD9\u4E2ACount\u6765\u6E90\u4E8Ereads\u6BD4\u5BF9\u5230reference genome \u5BF9\u5E94\u57FA\u56E0\u533A\u57DF\u7684reads\u6570\u91CF\uFF0C\u8FD9\u91CC\u6211\u4EEC\u4E0D\u8BA8\u8BBA\u53EF\u53D8\u526A\u5207\uFF0C\u6211\u4EEC\u8BA4\u4E3A\u57FA\u56E0\u65F6\u4E00\u4E2A\u5355\u5143\u3002\u5F53\u7136\uFF0C\u5728reads mapping\u7684\u65F6\u5019\u6211\u4EEC\u53EF\u80FD\u8003\u8651\u5230\uFF0C\u662F\u5426\u65F6\u552F\u4E00\u7684mapping\uFF0C\u8FD9\u4E9B\u5728\u8FD9\u91CC\u90FD\u5FFD\u7565\u3002\n    \n    \n    \u5F53\u6211\u4EEC\u8003\u8651count\u7684\u5206\u5E03\u65F6\uFF0C\u6211\u4EEC\u662F\u8BF4\u540C\u4E00\u7EC4\u5904\u7406\u6761\u4EF6\u4E0B\u7684gene\u90FD\u6709\u4E00\u4E2A\u5BF9\u5E94\u7684\u5206\u5E03\uFF0C\u56E0\u4E3A\u6BCF\u4E00\u4E2A\u57FA\u56E0\u90FD\u5F88\u72EC\u7279(\u6709\u81EA\u5DF1\u7684\u957F\u5EA6\u3001\u8868\u8FBE\u91CF)\uFF0C\u5F53\u6211\u4EEC\u8FDB\u884C\u5DEE\u5F02\u57FA\u56E0\u8868\u8FBE\u5206\u6790\u65F6\u6211\u4EEC\u662F\u5BF9\u6BCF\u4E00\u4E2A\u57FA\u56E0\u8FDB\u884C\u5047\u8BBE\u68C0\u9A8C\uFF0C\u5982\u679C\u5DEE\u5F02\u8868\u8FBE\u786E\u5B9E\u5B58\u5728\uFF0C\u90A3\u4E48\u4E24\u4E2A\u5904\u7406\u6761\u4EF6\u4E0B\u7684\u5206\u5E03\u5C06\u662F\u4E0D\u540C\u7684\u3002\n    \n    \n    \u6211\u4EEC\u53EF\u4EE5\u7528\u7B26\u53F7$X_{i1},...,X_{ij}\\sim Count Distribution$\u8868\u793A\u4E00\u4E2A\u6761\u4EF6\u4E0B\u7684\u4E00\u4E2A\u57FA\u56E0\u662F\u72EC\u7ACB\u540C\u5206\u5E03(Independent and identically distributed,iid)\n    \n    \n    \u5BF9\u4E8E\u8FD9\u4E2ACount Distribution\u6211\u4EEC\u53EF\u4EE5\u8003\u8651poisson \n  ", "oldCategory": null, "commentPath": null, "articleInComponentOrder": 0, "bilibili": "", "youtube": "", "video": "", "categoryPath": "html\/articleList", "categoryViewName": "202501655577", "isArticleDocLink": false, "users": null }
    var type="category"

    // var data = { "name":"哲学","parseType":"MARKDOWN","id": 891, "parentId": 0, "order": 3, "children": null, "lang": "ZH", "tags": [{ "id": 36, "parentId": 0, "order": 0, "createDate": "2023-10-25T23:53:20.000+08:00", "updateDate": "2023-10-25T23:53:20.000+08:00", "lang": "ZH", "langSource": null, "name": "\u8F6C\u5F55\u7EC4", "description": null, "slugName": "\u8F6C\u5F55\u7EC4", "enName": "202392520195", "key": null, "url": null }], "category": null, "formatContent": "\n<p>\u53C2\u8003<br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01c_RNAseq_count_distribution.html\">Gene-level differential expression analysis<\/a><br\/>+ <a href=\"https:\/\/uclouvain-cbio.github.io\/WSBIM2122\/sec-rnaseq.html\">Differential expression analysis<\/a><br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html\">https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html<\/a><br\/>+ <a href=\"https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html\">https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html<\/a><\/p>\n<p><a href=\"https:\/\/github.com\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"https:\/\/img.shields.io\/badge\/github-bioproj%2Fbioinfo-brightgreen?logo=github\"><\/a> <a href=\"https:\/\/colab.research.google.com\/github\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"\/badage\/colab-badge.svg\"><\/a><\/p>\n<p>## Count matrix<br\/>\u5F53\u6211\u4EEC\u9700\u8981\u5F00\u59CB\u8FDB\u884CRNA-seq\u6570\u636E\u5206\u6790\u4E4B\u524D\uFF0C\u6211\u4EEC\u4F1A\u5F97\u5230\u4E00\u4E2A\u57FA\u56E0\u6C34\u54C1\u7684\u8868\u8FBE\u77E9\u9635\uFF0C\u5176\u4E2D\u884C\u662F\u57FA\u56E0\uFF0C\u5217\u662F\u6837\u672C\uFF0C\u77E9\u9635\u4E2D\u7684\u6BCF\u4E2A\u503C\u90FD\u662FCounts(\u6B63\u6574\u6570)\u3002<\/p>\n<p><div class=\"pic-warp\"><div class=\"data-original row wp-90\"><img class=\"lazy\" data-original=\"http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png\" alt=\"\u751F\u4FE1\u5C0F\u6728\u5C4B\" \/><\/div><\/div><\/p>\n<p>\u50CFt-test\u8FD9\u7C7B\u578B\u7684\u68C0\u9A8C\u4E0D\u662F\u4E3Acounts\u8BBE\u8BA1\u7684\uFF0C\u5E76\u4E14\u6211\u4EEC\u5728\u5B9E\u9A8C\u8BBE\u8BA1\u65F6\u4E00\u7EC4\u7684\u91CD\u590D\u901A\u5E38\u4E0D\u662F\u5F88\u5927\uFF0C\u6211\u4EEC\u4E0D\u80FD\u76F8\u4FE1\u4E2D\u5FC3\u6781\u9650\u5B9A\u7406(Central Limit Theorem)\u53BB\u4F7F\u7528t-test\uFF0C\u56E0\u6B64\u6211\u4EEC\u9700\u8981\u5BF9counts\u8FDB\u884C\u5EFA\u6A21\u3002<\/p>\n<p>\u4E3A\u4E86\u65B9\u4FBF\u540E\u7EED\u8BA8\u8BBA\uFF0C\u6211\u5C06\u5148\u4ECB\u7ECD\u4E00\u4E9B\u7B26\u53F7<br\/>+ <span class=\"bioinfo-katex\">X_{ij}<\/span>\u8868\u793A<strong>gene i<\/strong> (i=1,...,m)\u5728<strong>sample j<\/strong> (j=1,...,n)\u4E2D\u7684Count\uFF0C\u8FD9\u4E2ACount\u6765\u6E90\u4E8Ereads\u6BD4\u5BF9\u5230reference genome \u5BF9\u5E94\u57FA\u56E0\u533A\u57DF\u7684reads\u6570\u91CF\uFF0C\u8FD9\u91CC\u6211\u4EEC\u4E0D\u8BA8\u8BBA\u53EF\u53D8\u526A\u5207\uFF0C\u6211\u4EEC\u8BA4\u4E3A\u57FA\u56E0\u65F6\u4E00\u4E2A\u5355\u5143\u3002\u5F53\u7136\uFF0C\u5728reads mapping\u7684\u65F6\u5019\u6211\u4EEC\u53EF\u80FD\u8003\u8651\u5230\uFF0C\u662F\u5426\u65F6\u552F\u4E00\u7684mapping\uFF0C\u8FD9\u4E9B\u5728\u8FD9\u91CC\u90FD\u5FFD\u7565\u3002<\/p>\n<p>\u5F53\u6211\u4EEC\u8003\u8651count\u7684\u5206\u5E03\u65F6\uFF0C\u6211\u4EEC\u662F\u8BF4\u540C\u4E00\u7EC4\u5904\u7406\u6761\u4EF6\u4E0B\u7684gene\u90FD\u6709\u4E00\u4E2A\u5BF9\u5E94\u7684\u5206\u5E03\uFF0C\u56E0\u4E3A\u6BCF\u4E00\u4E2A\u57FA\u56E0\u90FD\u5F88\u72EC\u7279(\u6709\u81EA\u5DF1\u7684\u957F\u5EA6\u3001\u8868\u8FBE\u91CF)\uFF0C\u5F53\u6211\u4EEC\u8FDB\u884C\u5DEE\u5F02\u57FA\u56E0\u8868\u8FBE\u5206\u6790\u65F6\u6211\u4EEC\u662F\u5BF9\u6BCF\u4E00\u4E2A\u57FA\u56E0\u8FDB\u884C\u5047\u8BBE\u68C0\u9A8C\uFF0C\u5982\u679C\u5DEE\u5F02\u8868\u8FBE\u786E\u5B9E\u5B58\u5728\uFF0C\u90A3\u4E48\u4E24\u4E2A\u5904\u7406\u6761\u4EF6\u4E0B\u7684\u5206\u5E03\u5C06\u662F\u4E0D\u540C\u7684\u3002<\/p>\n<p>\u6211\u4EEC\u53EF\u4EE5\u7528\u7B26\u53F7<span class=\"katex\">X_{i1},...,X_{ij}\\sim Count Distribution<\/span>\u8868\u793A\u4E00\u4E2A\u6761\u4EF6\u4E0B\u7684\u4E00\u4E2A\u57FA\u56E0\u662F\u72EC\u7ACB\u540C\u5206\u5E03(Independent and identically distributed,iid)<\/p>\n<p>\u5BF9\u4E8E\u8FD9\u4E2ACount Distribution\u6211\u4EEC\u53EF\u4EE5\u8003\u8651poisson<\/p>\n", "createDate": "2025-01-19T01:32:51.000+08:00", "updateDate": "2025-01-19T11:39:56.000+08:00", "templateName": "DEFAULT_COURSE_TEMPLATE", "status": "PUBLISHED", "likes": 0, "visits": 3, "commentNum": 0, "summary": "\u53C2\u8003+ Gene-level differential expression analysis+ Differential expression analysis+ https:\/\/hbctraini....", "title": "test", "viewName": "202501950638", "path": "html\/articleList", "picPath": "http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png", "picThumbPath": "", "pdfPath": null, "toc": "", "categoryId": 94, "tocJSON": "[]", "commentTemplateName": "DEFAULT_COMMENT", "openComment": true, "articleListSize": null, "isDesc": null, "linkPath": "\/articleList\/202501950638.html", "top": false, "cssClass": null, "isDivision": false, "tagIds": [36], "tocList": null, "isPublisher": false, "parentCategories": null, "forwardContentVO": null, "nextcontentVO": null, "css": "", "js": "", "originalContent": "  \n\n    \u53C2\u8003\n    + [Gene-level differential expression analysis](https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01c_RNAseq_count_distribution.html)\n    + [Differential expression analysis](https:\/\/uclouvain-cbio.github.io\/WSBIM2122\/sec-rnaseq.html)\n    + <https:\/\/hbctraining.github.io\/DGE_workshop\/lessons\/01_DGE_setup_and_overview.html>\n    + <https:\/\/hbctraining.github.io\/DGE_workshop_salmon_online\/lessons\/01b_DGE_setup_and_overview.html>\n    \n    \n    <a href=\"https:\/\/github.com\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"https:\/\/img.shields.io\/badge\/github-bioproj%2Fbioinfo-brightgreen?logo=github\"><\/a> <a href=\"https:\/\/colab.research.google.com\/github\/bioproj\/bioinfo\/blob\/master\/computational_biology\/computational_biology_count_distribution.ipynb\" target=\"_black\"><img alt=\"Static Badge\" src=\"\/badage\/colab-badge.svg\"><\/a>  \n    \n    ## Count matrix\n    \u5F53\u6211\u4EEC\u9700\u8981\u5F00\u59CB\u8FDB\u884CRNA-seq\u6570\u636E\u5206\u6790\u4E4B\u524D\uFF0C\u6211\u4EEC\u4F1A\u5F97\u5230\u4E00\u4E2A\u57FA\u56E0\u6C34\u54C1\u7684\u8868\u8FBE\u77E9\u9635\uFF0C\u5176\u4E2D\u884C\u662F\u57FA\u56E0\uFF0C\u5217\u662F\u6837\u672C\uFF0C\u77E9\u9635\u4E2D\u7684\u6BCF\u4E2A\u503C\u90FD\u662FCounts(\u6B63\u6574\u6570)\u3002\n    \n    ![html=(class=wp-90)\u751F\u4FE1\u5C0F\u6728\u5C4B](http:\/\/wangyang-bucket.oss-cn-beijing.aliyuncs.com\/cms\/image\/1736597956708_1736597887231.png)\n    \n    \u50CFt-test\u8FD9\u7C7B\u578B\u7684\u68C0\u9A8C\u4E0D\u662F\u4E3Acounts\u8BBE\u8BA1\u7684\uFF0C\u5E76\u4E14\u6211\u4EEC\u5728\u5B9E\u9A8C\u8BBE\u8BA1\u65F6\u4E00\u7EC4\u7684\u91CD\u590D\u901A\u5E38\u4E0D\u662F\u5F88\u5927\uFF0C\u6211\u4EEC\u4E0D\u80FD\u76F8\u4FE1\u4E2D\u5FC3\u6781\u9650\u5B9A\u7406(Central Limit Theorem)\u53BB\u4F7F\u7528t-test\uFF0C\u56E0\u6B64\u6211\u4EEC\u9700\u8981\u5BF9counts\u8FDB\u884C\u5EFA\u6A21\u3002\n    \n    \u4E3A\u4E86\u65B9\u4FBF\u540E\u7EED\u8BA8\u8BBA\uFF0C\u6211\u5C06\u5148\u4ECB\u7ECD\u4E00\u4E9B\u7B26\u53F7\n    + $X_{ij}$\u8868\u793A**gene i** (i=1,...,m)\u5728**sample j** (j=1,...,n)\u4E2D\u7684Count\uFF0C\u8FD9\u4E2ACount\u6765\u6E90\u4E8Ereads\u6BD4\u5BF9\u5230reference genome \u5BF9\u5E94\u57FA\u56E0\u533A\u57DF\u7684reads\u6570\u91CF\uFF0C\u8FD9\u91CC\u6211\u4EEC\u4E0D\u8BA8\u8BBA\u53EF\u53D8\u526A\u5207\uFF0C\u6211\u4EEC\u8BA4\u4E3A\u57FA\u56E0\u65F6\u4E00\u4E2A\u5355\u5143\u3002\u5F53\u7136\uFF0C\u5728reads mapping\u7684\u65F6\u5019\u6211\u4EEC\u53EF\u80FD\u8003\u8651\u5230\uFF0C\u662F\u5426\u65F6\u552F\u4E00\u7684mapping\uFF0C\u8FD9\u4E9B\u5728\u8FD9\u91CC\u90FD\u5FFD\u7565\u3002\n    \n    \n    \u5F53\u6211\u4EEC\u8003\u8651count\u7684\u5206\u5E03\u65F6\uFF0C\u6211\u4EEC\u662F\u8BF4\u540C\u4E00\u7EC4\u5904\u7406\u6761\u4EF6\u4E0B\u7684gene\u90FD\u6709\u4E00\u4E2A\u5BF9\u5E94\u7684\u5206\u5E03\uFF0C\u56E0\u4E3A\u6BCF\u4E00\u4E2A\u57FA\u56E0\u90FD\u5F88\u72EC\u7279(\u6709\u81EA\u5DF1\u7684\u957F\u5EA6\u3001\u8868\u8FBE\u91CF)\uFF0C\u5F53\u6211\u4EEC\u8FDB\u884C\u5DEE\u5F02\u57FA\u56E0\u8868\u8FBE\u5206\u6790\u65F6\u6211\u4EEC\u662F\u5BF9\u6BCF\u4E00\u4E2A\u57FA\u56E0\u8FDB\u884C\u5047\u8BBE\u68C0\u9A8C\uFF0C\u5982\u679C\u5DEE\u5F02\u8868\u8FBE\u786E\u5B9E\u5B58\u5728\uFF0C\u90A3\u4E48\u4E24\u4E2A\u5904\u7406\u6761\u4EF6\u4E0B\u7684\u5206\u5E03\u5C06\u662F\u4E0D\u540C\u7684\u3002\n    \n    \n    \u6211\u4EEC\u53EF\u4EE5\u7528\u7B26\u53F7$X_{i1},...,X_{ij}\\sim Count Distribution$\u8868\u793A\u4E00\u4E2A\u6761\u4EF6\u4E0B\u7684\u4E00\u4E2A\u57FA\u56E0\u662F\u72EC\u7ACB\u540C\u5206\u5E03(Independent and identically distributed,iid)\n    \n    \n    \u5BF9\u4E8E\u8FD9\u4E2ACount Distribution\u6211\u4EEC\u53EF\u4EE5\u8003\u8651poisson \n  ", "oldCategory": null, "commentPath": null, "articleInComponentOrder": 0, "bilibili": "", "youtube": "", "video": "", "categoryPath": "html\/articleList", "categoryViewName": "202501655577", "isArticleDocLink": false, "users": null }
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